Part:BBa_K766004:Design

AndNot gate based on AHL

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 943
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 385
  Illegal AgeI site found at 582
  Illegal AgeI site found at 1630
  Illegal AgeI site found at 1742
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2859
  

Design Notes

We want to construct a logic gate in our project.

Source

We use several part from igem kit to get each gene like cI and different promoter. Then we use overlap PCR to link them together.